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human genome wide crispr cas9 knockout pooled library geckov2  (Addgene inc)


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    Addgene inc human genome wide crispr cas9 knockout pooled library geckov2
    In vivo genome-wide CRISPR screening in PDAC. a Schematic representation of the loss-of-function genome-wide screen using the human <t>lentiviral</t> <t>CRISPR/Cas9</t> library <t>(GeCKOv2)</t> library A in pancreatic ductal adenocarcinoma cell line (HPAF-II). b Average mass of extracted tumor from NSG mice subcutaneous transplanted with 30 million cells and grown for 28 days. Mean of three independent infection replicate experiments ( n = 5, 1 mouse per biological replicate was randomly selected for deep sequencing). Data are represented as mean ± SEM. c Normalized read count distribution from sequenced amplicons. d The unmapped percentage of sgRNAs in the library in cells before transplantation ( n = 3), and tumor samples ( n = 3) on day 28. e Statistical dispersion graphic (Gini index) of the sgRNA distribution within samples from cells and tumor replicates. f Cumulative distribution function (CDF) of library sgRNAs in the cell representation and tumor sample replicates. Shifts in tumor samples reflect altered read counts in subset of sgRNAs. g Pearson correlation of the sgRNA reads between all samples from in vitro and in vivo
    Human Genome Wide Crispr Cas9 Knockout Pooled Library Geckov2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 226 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+genome+wide+crispr+cas9+knockout+pooled+library+geckov2/Human+CRISPR+Knockout+Pooled+Library+(GeCKO+v2)+(Pooled+Library+%231000000048%2C+%231000000049)/pmc12973555-416-0-13
    Average 96 stars, based on 226 article reviews
    human genome wide crispr cas9 knockout pooled library geckov2 - by Bioz Stars, 2026-10
    96/100 stars

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    1) Product Images from "Identification of HSPE1 as a new actionable cancer vulnerability leads to an innovative and effective combination therapy for pancreatic ductal adenocarcinoma"

    Article Title: Identification of HSPE1 as a new actionable cancer vulnerability leads to an innovative and effective combination therapy for pancreatic ductal adenocarcinoma

    Journal: Molecular Cancer

    doi: 10.1186/s12943-026-02587-9

    In vivo genome-wide CRISPR screening in PDAC. a Schematic representation of the loss-of-function genome-wide screen using the human lentiviral CRISPR/Cas9 library (GeCKOv2) library A in pancreatic ductal adenocarcinoma cell line (HPAF-II). b Average mass of extracted tumor from NSG mice subcutaneous transplanted with 30 million cells and grown for 28 days. Mean of three independent infection replicate experiments ( n = 5, 1 mouse per biological replicate was randomly selected for deep sequencing). Data are represented as mean ± SEM. c Normalized read count distribution from sequenced amplicons. d The unmapped percentage of sgRNAs in the library in cells before transplantation ( n = 3), and tumor samples ( n = 3) on day 28. e Statistical dispersion graphic (Gini index) of the sgRNA distribution within samples from cells and tumor replicates. f Cumulative distribution function (CDF) of library sgRNAs in the cell representation and tumor sample replicates. Shifts in tumor samples reflect altered read counts in subset of sgRNAs. g Pearson correlation of the sgRNA reads between all samples from in vitro and in vivo
    Figure Legend Snippet: In vivo genome-wide CRISPR screening in PDAC. a Schematic representation of the loss-of-function genome-wide screen using the human lentiviral CRISPR/Cas9 library (GeCKOv2) library A in pancreatic ductal adenocarcinoma cell line (HPAF-II). b Average mass of extracted tumor from NSG mice subcutaneous transplanted with 30 million cells and grown for 28 days. Mean of three independent infection replicate experiments ( n = 5, 1 mouse per biological replicate was randomly selected for deep sequencing). Data are represented as mean ± SEM. c Normalized read count distribution from sequenced amplicons. d The unmapped percentage of sgRNAs in the library in cells before transplantation ( n = 3), and tumor samples ( n = 3) on day 28. e Statistical dispersion graphic (Gini index) of the sgRNA distribution within samples from cells and tumor replicates. f Cumulative distribution function (CDF) of library sgRNAs in the cell representation and tumor sample replicates. Shifts in tumor samples reflect altered read counts in subset of sgRNAs. g Pearson correlation of the sgRNA reads between all samples from in vitro and in vivo

    Techniques Used: In Vivo, Genome Wide, CRISPR, Infection, Sequencing, Transplantation Assay, Dispersion, In Vitro

    Related Articles

    Genome Wide:

    Article Title: Identification of HSPE1 as a new actionable cancer vulnerability leads to an innovative and effective combination therapy for pancreatic ductal adenocarcinoma
    Article Snippet: .. Human genome-wide CRISPR/cas9 knockout pooled library GeCKOv2 was a gift from Feng Zhang (Addgene#1,000,000,048). ..

    Article Title: In vivo genome-wide CRISPR screen reveals breast cancer vulnerabilities and synergistic mTOR/Hippo targeted combination therapy
    Article Snippet: The modifications (the insertion, deletion, or mismatched DNA) of the interested region from the PCR products were then cleaved and detected by the Detection Enzyme from GeneArt Genomic Cleavage Detection Kit. .. Human genome-wide CRISPR/cas9 knockout pooled library GeCKOv2 was a gift from Feng Zhang (Addgene#1000000048). .. The amplification and virus production of GeCKOv2 library A were performed as described in the Addgene protocol .

    Article Title: Identification of HSPE1 as a new actionable cancer vulnerability leads to an innovative and effective combination therapy for pancreatic ductal adenocarcinoma.
    Article Snippet: .. Human genome-wide CRISPR/cas9 knockout pooled library GeCKOv2 was a gift from Feng Zhang (Addgene#1000000048). ..

    CRISPR:

    Article Title: Identification of HSPE1 as a new actionable cancer vulnerability leads to an innovative and effective combination therapy for pancreatic ductal adenocarcinoma
    Article Snippet: .. Human genome-wide CRISPR/cas9 knockout pooled library GeCKOv2 was a gift from Feng Zhang (Addgene#1,000,000,048). ..

    Article Title: In vivo genome-wide CRISPR screen reveals breast cancer vulnerabilities and synergistic mTOR/Hippo targeted combination therapy
    Article Snippet: The modifications (the insertion, deletion, or mismatched DNA) of the interested region from the PCR products were then cleaved and detected by the Detection Enzyme from GeneArt Genomic Cleavage Detection Kit. .. Human genome-wide CRISPR/cas9 knockout pooled library GeCKOv2 was a gift from Feng Zhang (Addgene#1000000048). .. The amplification and virus production of GeCKOv2 library A were performed as described in the Addgene protocol .

    Article Title: Identification of HSPE1 as a new actionable cancer vulnerability leads to an innovative and effective combination therapy for pancreatic ductal adenocarcinoma.
    Article Snippet: .. Human genome-wide CRISPR/cas9 knockout pooled library GeCKOv2 was a gift from Feng Zhang (Addgene#1000000048). ..

    Knock-Out:

    Article Title: Identification of HSPE1 as a new actionable cancer vulnerability leads to an innovative and effective combination therapy for pancreatic ductal adenocarcinoma
    Article Snippet: .. Human genome-wide CRISPR/cas9 knockout pooled library GeCKOv2 was a gift from Feng Zhang (Addgene#1,000,000,048). ..

    Article Title: In vivo genome-wide CRISPR screen reveals breast cancer vulnerabilities and synergistic mTOR/Hippo targeted combination therapy
    Article Snippet: The modifications (the insertion, deletion, or mismatched DNA) of the interested region from the PCR products were then cleaved and detected by the Detection Enzyme from GeneArt Genomic Cleavage Detection Kit. .. Human genome-wide CRISPR/cas9 knockout pooled library GeCKOv2 was a gift from Feng Zhang (Addgene#1000000048). .. The amplification and virus production of GeCKOv2 library A were performed as described in the Addgene protocol .

    Article Title: Identification of HSPE1 as a new actionable cancer vulnerability leads to an innovative and effective combination therapy for pancreatic ductal adenocarcinoma.
    Article Snippet: .. Human genome-wide CRISPR/cas9 knockout pooled library GeCKOv2 was a gift from Feng Zhang (Addgene#1000000048). ..



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    Image Search Results


    In vivo genome-wide CRISPR screening in PDAC. a Schematic representation of the loss-of-function genome-wide screen using the human lentiviral CRISPR/Cas9 library (GeCKOv2) library A in pancreatic ductal adenocarcinoma cell line (HPAF-II). b Average mass of extracted tumor from NSG mice subcutaneous transplanted with 30 million cells and grown for 28 days. Mean of three independent infection replicate experiments ( n = 5, 1 mouse per biological replicate was randomly selected for deep sequencing). Data are represented as mean ± SEM. c Normalized read count distribution from sequenced amplicons. d The unmapped percentage of sgRNAs in the library in cells before transplantation ( n = 3), and tumor samples ( n = 3) on day 28. e Statistical dispersion graphic (Gini index) of the sgRNA distribution within samples from cells and tumor replicates. f Cumulative distribution function (CDF) of library sgRNAs in the cell representation and tumor sample replicates. Shifts in tumor samples reflect altered read counts in subset of sgRNAs. g Pearson correlation of the sgRNA reads between all samples from in vitro and in vivo

    Journal: Molecular Cancer

    Article Title: Identification of HSPE1 as a new actionable cancer vulnerability leads to an innovative and effective combination therapy for pancreatic ductal adenocarcinoma

    doi: 10.1186/s12943-026-02587-9

    Figure Lengend Snippet: In vivo genome-wide CRISPR screening in PDAC. a Schematic representation of the loss-of-function genome-wide screen using the human lentiviral CRISPR/Cas9 library (GeCKOv2) library A in pancreatic ductal adenocarcinoma cell line (HPAF-II). b Average mass of extracted tumor from NSG mice subcutaneous transplanted with 30 million cells and grown for 28 days. Mean of three independent infection replicate experiments ( n = 5, 1 mouse per biological replicate was randomly selected for deep sequencing). Data are represented as mean ± SEM. c Normalized read count distribution from sequenced amplicons. d The unmapped percentage of sgRNAs in the library in cells before transplantation ( n = 3), and tumor samples ( n = 3) on day 28. e Statistical dispersion graphic (Gini index) of the sgRNA distribution within samples from cells and tumor replicates. f Cumulative distribution function (CDF) of library sgRNAs in the cell representation and tumor sample replicates. Shifts in tumor samples reflect altered read counts in subset of sgRNAs. g Pearson correlation of the sgRNA reads between all samples from in vitro and in vivo

    Article Snippet: Human genome-wide CRISPR/cas9 knockout pooled library GeCKOv2 was a gift from Feng Zhang (Addgene#1,000,000,048).

    Techniques: In Vivo, Genome Wide, CRISPR, Infection, Sequencing, Transplantation Assay, Dispersion, In Vitro